I'm wondering if you could help me out. I have a colony of camponotus vagus with 3 workers in a test tube sat inside a foraging box. The problem is that for about a month now she has not laid any eggs and I haven't seen them eat anything. They are kept at around 24 degrees Celsius and usually I take a peek every 3 days.
Not sure where to go with this colony now so if you could help it would be appreciated.
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I had the same problem for ages with my C. ligniperda. My queen has only laid eggs very recently, and the key seems to be keep them in a dark place with a good supply of insect food if you can. If the water in the test tube starts to dry out, then move them into a clean new one with a fresh supply of water. Also feed the adults with honey, sugar water and maybe spray a tiny amount of water inside the foraging box if the weather is hot to create some humidity.
I don't have any experience or knowledge of this species but I do keep Ligniperdus and it took a few months to get the colony going. Basically I also had a queen and 3 workers not really doing much. Make sure the colony has a test tube nest set up available, in a small outworld with food, in a dark undisturbed place that's warm (I use my loft). Some species have stopped brood production by now due to the time of year but I am not sure if its relevant to your colony.